fam dye wavelength

<]/Prev 83581/XRefStm 1404>> 0000033960 00000 n 0000046783 00000 n Choose dyes with appropriate excitation wavelengths and little to no overlap in their emission spectra. Spectral Properties . FAM is the most popular of these dyes, and often provides greater assay sensitivity than some of the other dyes available. endstream endobj 117 0 obj <>/Filter/FlateDecode/Index[8 68]/Length 21/Size 76/Type/XRef/W[1 1 1]>>stream 0000004754 00000 n Table 1 provides a list of reporter dyes compatible with common instrumentation. 0000034281 00000 n 0000010927 00000 n Fluorescence emission wavelengths used for multiplex real-time PCR. 0000049526 00000 n Oregon Green. TAMRA Absorbance. 0000048744 00000 n 0000042037 00000 n TAMRA Emission. For example, FAM is a good dye choice to detect low-copy transcripts because it has high ï¬uorescent signal intensity. 0000025302 00000 n lists many of the fluorescent dyes currently available with their excitation and emission wavelengths and their most optimal quencher option. xref There is an increase of signal at 670 nm when TdT is present. 0000001090 00000 n Below pH 7, 6-FAMâ¢ becomes protonated, which results in decreased fluorescence. FAM Emission. These four reporter dyes with distinct spectra are optimized to work together with minimal spectral overlap (Figure 2). Wavelength (nm) FAM Absorption and Emission Spectra . four reporter dyes, a new quencher, and a new master mix. All four dyes can be excited at a single wavelength (488 nm), but emit at distinctly different wavelengths. 0000025244 00000 n 0000017605 00000 n 0000002106 00000 n Single dyes such as FITC, PE, APC and PerCP have been available for many years, but there are now alternatives available from Alexa Fluor dyes, which offer users greater photostability and brighter fluorescence. Performance without compromise FAM 494 519 Fluorescein Iso-thiocyanate (FITC) 495 519 488 ... Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. 0000001284 00000 n 0000002553 00000 n This suggests successful detection of active TdT using FAM and Cy5 FRET pairs. This structure features a linker between Coumarin 343 fluorophore, and the functional group. Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). It is widely used as a fluorescent tracer for many applications. NBD-X. 0000017792 00000 n trailer 0000021999 00000 n It is typically used in the pH range 7.5-8.5. 0000034128 00000 n H��Y�%��n��Έ�A<3B��AƘq��Hh4��D՝i/�з"+32�'��O߿~z�>��_�J%}��W/��K�^��m�"�cߔ�j��^�|[ҫ_.��^��/גK��·ߓy�J3�}�. Many different fluorophores can be readily attached to oligonucleotides and they form the basis of the detection systems used in DNA sequencing, forensic science and genetic analysis. 0000051199 00000 n 0000011845 00000 n 0000006899 00000 n 0000069862 00000 n Refer to your instrument manufacturerâs guidelines for information specific to your particular instrument. %PDF-1.4 %�� (B) Emission wavelength scan with 2bA FAM oligo and Cy5-dCTP with TdT. 0000005860 00000 n 0000001404 00000 n 466. 0000014285 00000 n Wavelength scan from 480 nm to 700 nm with excitation at 450 nm. trailer <]>> startxref 0 %%EOF 36 0 obj<>stream The absorption spectrum of a compound thus is the ï¬rst expression of possible photoactivity. endstream endobj 77 0 obj <>/Metadata 6 0 R/Outlines 1 0 R/PageLayout/SinglePage/Pages 5 0 R/StructTreeRoot 8 0 R/Type/Catalog>> endobj 78 0 obj <>/ExtGState<>/Font<>/XObject<>>>/Rotate 0/StructParents 0/Type/Page>> endobj 79 0 obj <> endobj 80 0 obj [/Indexed/DeviceRGB 1 93 0 R] endobj 81 0 obj <>stream Fast Real-Time PCR System. The donor dye (fluorescein) is excited by the blue LED. 0000024631 00000 n 0000026489 00000 n Wavelength (nm) TAMRA Absorption and Emission Spectra . It is available as a dark orange/red powder slightly soluble in water and alcohol. Many derivatives of fluorescein are used in research, including FITC, FAM, and carboxyfluorescein. Following absorption, ï¬uorescence emission may be of central importance as occurs in various ï¬uorometric analytical procedures (e.g. A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. 0000078488 00000 n 500. It can be excited using a 640 nm laser paired with a 670/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAriaâ¢ Fusion. Live Chat Support Software ... FAM. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. I think one of the most common fluorophores for the Taqman probe is FAM with an emission wavelength 520 nm and TET with 536 nm. 0000003970 00000 n 0000000016 00000 n 0000051916 00000 n 0000011458 00000 n 0000001164 00000 n 0000077868 00000 n x�b``�f``>� q-��Y80() �JC1�/��:�sG0�n$,h��d!��gÎd ��8�� z�E�JK!�� f�� endstream endobj 8 0 obj<> endobj 9 0 obj<>/Encoding<>>>>> endobj 10 0 obj<>/Font<>/ProcSet[/PDF/Text]/ExtGState<>>>/Type/Page>> endobj 11 0 obj<> endobj 12 0 obj<> endobj 13 0 obj<>stream 0000046280 00000 n 0000007112 00000 n Wavelength (nm) 350 400 450 500 550 600 650 700 For more information on Bio-Rad imaging products, contact your local Bio-Rad representative. dye. 0000030517 00000 n 520. 0000017395 00000 n 0000025945 00000 n TET. Lifetime Data of Selected Fluorophores. 6-FAMâ¢ is the most commonly used fluorescent dye for attachment to oligonucleotides and is compatible with most fluorescence detection equipm ent. 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